Overexpression of Bacterial Hemoglobin Causes Incorporation of Pre-β-Lactamase into Cytoplasmic Inclusion Bodies
- Creators
- Rinas, Ursula
- Bailey, James E.
Abstract
The expression of Vitreoscilla hemoglobin (VHb) in Escherichia coli JM101 (pRED2) causes the incorporation of the TEM beta-lactamase precursor into cytoplasmic inclusion bodies (IBs). Less pre-beta-lactamase is translocated and processed to its mature, periplasmic form in the strain coexpressing VHb than in the control strain E. coli JM101(pUC19) not expressing VHb. When cells are grown in a special fed-batch procedure, the formation of cytoplasmic IBs consisting of pre-beta-lactamase is also inducible in the control strain. Comparative microscopic and compositional analyses of IBs generated in E. coli JM101(pUC19) and JM101(pRED2) under identical growth conditions strongly suggest that pre-beta-lactamase and VHb coaggregate into common IBs in E. coli JM101 (pRED2).
Additional Information
Copyright © 1993 by the American Society for Microbiology. Received 14 April 1992/Accepted 21 November 1992 We are grateful to R. A. Hart for assistance and many fruitful discussions, C. Khosla for providing the plasmid pRED2, J. Richards for providing serum directed against TEM β-lactamase, J. Eden for assistance in electron microscopy, and P. Martinez for help with plasmid preparation. This work was supported by a postdoctoral fellowship from the Deutscher Akademischer Austauschdienst (to U.R.) and by the National Science Foundation (grant CBT8721973).Files
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Additional details
- Eprint ID
- 5735
- Resolver ID
- CaltechAUTHORS:RINaem93
- Created
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2006-10-30Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field