Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published April 1977 | Published
Journal Article Open

Properties of an antiserum against native dynein 1 from sea urchin sperm flagella

Abstract

Effects of an antiserum against native dynein 1 from sperm flagella of the sea urchin Strongylocentrotus purpuratus were compared with effects of an antiserum previously obtained against an ATPase-active tryptic fragment (fragment 1A) of dynein 1 from sperm flagella of the sea urchin, Anthocidaris crassispina. Both antisera precipitate dynein 1 and do not precipitate dynein 2. Only the fragment 1A antiserum precipitates fragment 1A and produces a measurable inhibition of dynein 1 ATPase activity. Both antisera inhibit the movement and the movement- coupled ATP dephosphorylation of reactivated spermatozoa. The inhibition of movement by the antiserum against dynein 1 is much less than by the antiserum against fragment 1A, suggesting that a specific interference with the active ATPase site may be required for effective inhibition of movement. Both antisera reduce the bend angle as well as the beat frequency of reactivated S. purpuratus spermatozoa, suggesting that the bend angle may depend on the activity of the dynein arms which generate active sliding.

Additional Information

© 1977 Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 28 September 1976, and in revised form 7 December 1976. We thank Tom Simonick and Eileen Roy for able assistance with portions of this work; Professor Ray Owen for immunology laboratory facilities and advice; and the U.S. Public Health Service for support provided by National Institutes of Health research grant GM 18711.

Attached Files

Published - OGAjcb77.pdf

Files

OGAjcb77.pdf
Files (1.5 MB)
Name Size Download all
md5:4816a731ca42afc93d52308da05bf315
1.5 MB Preview Download

Additional details

Created:
August 22, 2023
Modified:
October 17, 2023