Heavy chain genes of rabbit IgG: isolation of a cDNA encoding gamma heavy chain and identification of two genomic C gamma genes

Creators: Martens, Christine L.; Moore, Kevin W.; Steinmetz, Michael; Hood, Leroy; Knight, Katherine L.

Abstract

A cDNA library was constructed by using rabbit spleen poly(A)+RNA as template, and from this library was isolated a cDNA clone, p2a2, that encodes 179 amino acids of the heavy chain of rabbit IgG. The nucleotide sequence of p2a2 showed that it encodes the COOH-terminal eight amino acids of the CH1 domain, the hinge region, the CH2 domain, and the NH2-terminal half of the CH3 domain of C gamma. Southern blot hybridization analysis of rabbit sperm DNA showed that two EcoRI fragments hybridized strongly with the C gamma cDNA. The p2a2 cDNA was used as a probe to isolate recombinant Charon 4A phage clones containing C gamma sequences from a genomic library of rabbit liver DNA. Two distinct DNA segments were identified by restriction mapping and hybridization analysis, suggesting that the haploid rabbit genome may contain two different C gamma genes.

Additional Information

© 1982 by the National Academy of Sciences. Communicated by Felix Haurowitz, July 6, 1982. We thank Drs. Edward Mocarski and Kiranur Subramanian for help with nucleotide sequence analysis. This work was supported by U.S. Public Health Service Grants AI 11234, AI 17853, and GM 06965. M.S. is the recipient of a fellowship from the Deutsche Forschungsgemeinschaft. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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