Published December 15, 2008
| Published
Journal Article
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Microfluidic polymerase chain reaction
Abstract
We implement microfluidic technology to miniaturize a thermal cycling system for amplifying DNA fragments. By using a microfluidic thermal heat exchanger to cool a Peltier junction, we have demonstrated rapid heating and cooling of small volumes of solution. We use a miniature K-type thermocouple to provide a means for in situ sensing of the temperature inside the microrefrigeration system. By combining the thermocouple, two power supplies controlled by a relay system, and computer automation, we reproduce the function of a commercial polymerase chain reaction thermal cycler and demonstrate amplification of a DNA sample of about 1000 base pairs.
Additional Information
© 2008 American Institute of Physics. Received 4 July 2008; accepted 12 November 2008; published 16 December 2008. The authors gratefully acknowledge financial support for this research by grants from the National Science Foundation (Grant Nos. CHE-0515363 and DMR-0351848).Attached Files
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Additional details
- Eprint ID
- 12688
- Resolver ID
- CaltechAUTHORS:MALapl08
- CHE-0515363
- National Science Foundation
- DMR-0351848
- National Science Foundation
- Created
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2008-12-19Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field