DNA sequences of the repressor gene and operator region of bacteriophage P2
Abstract
The nucleotide sequence of the repressor gene C of the temperate phage P2 has been determined. It codes for a nonbasic polypeptide, 99 amino acids long. Twelve repressor-defective mutants have been mapped. All but one are located within the presumed coding part of the gene. There is a strong promoter sequence and an 8-base-pair inverted repeat preceding the gene. The P2 repressor protein shows structural similarity to other DNA-binding proteins. The operator region for the early replication functions was located by sequencing the DNA of three virulent mutants. The sequence indicates that there are two repressor-binding sites. In addition, one of the sites shows sequence homology with part of the operator region of the biotin operon of Escherichia coli.
Additional Information
© 1984 by the National Academy of Sciences. Communicated by Norman H. Horowitz, March 12, 1984. We would like to thank Peter Alestrom for advice on the DNA sequencing and G. Bertani for helpful discussions. A large part of the DNA sequencing was done in the Department of Microbiology at the University of Uppsala, Uppsala, Sweden. This work was supported by Grant 72 from the Swedish Medical Research Council. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
Published - LJUpnas84.pdf
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Additional details
- PMCID
- PMC345353
- Eprint ID
- 7254
- Resolver ID
- CaltechAUTHORS:LJUpnas84
- 72
- Swedish Medical Research Council
- Created
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2007-01-24Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field