Strategies for studying permeation at voltage-gated ion channels
- Creators
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Lester, Henry A.
Abstract
Voltage-dependent ion channels are presently thought to consist of several distinct functional regions: (a) activation gates, (b) inactivation gates, and permeation pathways. This chapter focuses on permeation pathways and may spur new ideas about experiments that use site-directed mutagenesis to probe the ion conduction pathway. Some hubris is required to attempt a survey of this field since individual families -- K^+, Na^+, or Ca^(2+) -- have been reviewed in detail (15, 68, 115, 127). My unified treatment is motivated by the structural similarity suggested by recent cDNA sequencing data on this group (see, for instance, 24). There have been many excellent previous treatments of ion channel permeation (6, 15, 34, 35, 51, 53, 68, 73, 74, 115, 127).
Additional Information
"Reprinted, with permission, from the Annual Review of Physiology, Volume 53 copyright 1991 by Annual Reviews, www.annualreviews.org". I thank R. Aldrich and R. MacKinnon for sharing their work prior to publication, P. Lazarow for challenges on rectification, and N. Davidson, B. Hille, T. Begenisich, and members of my research group for many helpful comments. Preparation of this review was supported by grants from the Klingenstein Foundation, the Muscular Dystrophy Association, and the National Institutes of Health (GM-29836 and NS-11756).Attached Files
Published - LESarp91.pdf
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Additional details
- Eprint ID
- 9487
- Resolver ID
- CaltechAUTHORS:LESarp91
- Klingenstein Foundation
- Muscular Dystrophy Association
- GM-29836
- NIH
- NS-11756
- NIH
- Created
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2008-01-25Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field