Structure of a nicked DNA-protein complex isolated from simian virus 40: Covalent attachment of the protein to DNA and nick specificity
- Creators
- Kasamatsu, Harumi
- Wu, Madeline
Abstract
A portion of the nicked circular DNA isolated from purified simian virus 40 contains a protein-DNA complex in which protein(s) is covalently attached to the end of a DNA single strand. (Nicked DNA is double-stranded DNA that contains at least one single-strand scission.) The protein was visualized by electron microscopy and labeled in vitro with 125I. The bond between the protein and the DNA is stable in alkali, 4 M guanidine· hydrochloride, 3.86 M hydroxylamine (pH 4.23), and in 98% formamide. Most of the molecules in the nicked circular DNA fraction contained one nick. The nick occurs on either of the two complementary strands; the specific nick sites on the two strands are staggered, but lie within a few hundred nucleotides of each other.
Additional Information
Copyright © 1976 by the National Academy of Sciences. Communicated by Jerome Vinograd, April 5, 1976. We are indebted to Profs. J. Vinograd and N. Davidson, in whose laboratories this work was conducted, for advice, encouragement, and criticism. This research has been supported by National Institutes of Health Grants CA08014, GM20927, and GM10991.Files
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Additional details
- Eprint ID
- 9050
- Resolver ID
- CaltechAUTHORS:KASpnas76
- Created
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2007-10-23Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field