Published October 12, 1987
| Published
Journal Article
Open
Adenine specific DNA chemical sequencing reaction
- Creators
- Iverson, Brent L.
-
Dervan, Peter B.
Abstract
Reaction of DNA with K2PdCI4 at pH2.0 followed by a piperidine workup produces specific cleavage at adenine (A) residues. Product analysis revealed the K2PdCI4 reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions. Adenine residues methylated at the exocyclic amine (N6) react with lower efficiency than unmethylated adenine in an identical sequence. This simple protocol specific for A may be a useful addition to current chemical sequencing reactions.
Additional Information
Copyright © 1997 Oxford University Press. Received July 13, 1987. Accepted August 30, 1987. We are grateful to the American Cancer Society (NP 428) for support of this research and for a National Research Service Award to B.L.I. from the National Institute of General Medical Sciences.Attached Files
Published - IVEnar97.pdf
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IVEnar97.pdf
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Additional details
- PMCID
- PMC306310
- Eprint ID
- 4010
- Resolver ID
- CaltechAUTHORS:IVEnar87
- NP-428
- American Cancer Society
- NIH Predoctoral Fellowship
- Created
-
2006-07-24Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field