Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published April 1950 | public
Journal Article Open

A colorimetric method for the determination of chymotrypsin activity

Abstract

The L isomers of esters, amides, and hydrazides of the general formula RCONHCHR1COR2 where R = CH3-, C6H5-, etc., R1 = C6H6CH2-, p-HO-C6H4CH2-, etc., and R2 = -OCH3, -NH2, or -NHNH2, are known to be hydrolyzed by chymotrypsin (l-6). It has now been found that the corresponding hydroxamides, RCONHCHR1CONHOH, where R1 = C6H5CH2-, are also hydrolyzed by this enzyme (Table I). Aside from the obvious usefulness of the above hydroxamides in the further definition of chymotrypsin activity we wish to point out that these latter substrates provide the basis for a simple, sensitive, and rapid calorimetric method for the determination of chymotrypsin activity which can be extended to a number of other proteolytic enzymes.

Additional Information

Copyright © 1950 by the American Society of Biological Chemists (Received for publication, October 31, 1949) Supported in part by a grant from Eli Lilly and Company. Contribution No. 1348 from the Gates and Crellin Laboratories of Chemistry, California Institution of Technology, Pasadena

Files

ISEjbc50b.pdf
Files (307.8 kB)
Name Size Download all
md5:9a5de67d393ee07efe6c6f2321b27a9b
307.8 kB Preview Download

Additional details

Created:
August 21, 2023
Modified:
October 16, 2023