alphabeta sequence of F is IS31
Abstract
Previous studies have shown that there is a deoxyribonucleic acid (DNA) segment, of length 1.3 kb and denoted as the alphabeta sequence, which occurs twice on the F plasmid at corrdinates 93.2 to 94.5/OF kb and 13.7 to 15.0F kb. In the present investigation, heteroduplexes were prepared between a phage DNA carrying the insertion sequence IS3 and suitable F-prime DNAs. The hybrids formed show that IS3 is the same as alphabeta. This result plus previous studies support the view that: (i) the insertion sequence IS2 and IS3 occur on F and, in multiple copies, on the main bacterial chromosome of Escherichia coli K-12; and (ii)these IS sequences on the main bacterial chromosomes are hot spots for Hfr formation by reciprocal recombination with the corresponding sequences of F.
Additional Information
Copyright © 1975 by the American Society for Microbiology. Received for publication 31 March 1975 We again acknowledge the gift of the phage [lambda]placMS505 from M. Malamy. This research has been supported by Public Health Service grants GM 10991 from the National Institute of General Medical Sciences and AI-08619 (from the National Institute of Allergy and Infectious Diseases), GB-30 581 from the National Science Foundation, and VC-139 from the American Cancer Society. Kay Ptashne is the recipient of a postdoctoral research fellowship (SF02ES54436) from National Institute of Environmental Health Sciences. Contribution no. 5083 from the Department of Chemistry of the California Institute of Technology.Files
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Additional details
- Eprint ID
- 2212
- Resolver ID
- CaltechAUTHORS:HUSjbact75c
- Created
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2006-03-15Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field