Published July 1, 1975
| public
Journal Article
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Establishment of tight junctions between epithelial cells
- Creators
- Hudspeth, A. J.
Abstract
Epithelia serve as barriers to the diffusion of solutes between body compartments, and must do so despite the frequent loss of cells. When single cells are experimentally removed from the Necturus gallbladder epithelium, contiguous cells migrate to fill the defect within 30 min. Electrophysiological measurements show that the local electrical resistance across the epithelium in the region of a wound returns to normal in the same period of time; electron microscopy demonstrates that tight junctions are formed concurrently. Physiologically functional and morphologically recognizable tight junctions can thus be established within 30 min, demonstrating a mechanism for the rapid restoration of epithelial integrity after cell loss.
Additional Information
© 1975 by the National Academy of Sciences. Communicated by Stephen W. Kuffler, April 22, 1975. I thank Drs. B. Forslind, E.A. Kravitz, U.J. McMahan, and T.N. Wiesel for the use of equipment, Dr. A.G. Yee for assistance in freeze-fracturing, and Drs. S.W. Kuffler, E. Raviola, and A.E. Stuart for comments on the manuscript. This research was supported in part by USPHS Grant MH-07084 and by a fellowship from the Alfred P. Sloan Foundation.Files
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Additional details
- Eprint ID
- 7619
- Resolver ID
- CaltechAUTHORS:HUDpnas75
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2007-03-14Created from EPrint's datestamp field
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2021-11-08Created from EPrint's last_modified field