Isolation and characterization of the dopa decarboxylase gene of Drosophila melanogaster

Creators: Hirsh, Jay; Davidson, Norman

Abstract

We have isolated chromosomal deoxyribonucleic acid clones containing the Drosophila dopa decarboxylase gene. We describe an isolation procedure which can be applied to other nonabundantly expressed Drosophila genes. The dopa decarboxylase gene lies within or very near polytene chromosome band 37C1-2. The gene is interrupted by at least one intron, and the primary mode of regulation is pretranslational. At least two additional sequences hybridized by in vivo ribonucleic acid-derived probes are found within a 35-kilobase region surrounding the gene. The developmental profile of ribonucleic acid transcribed from one of these regions differs from that of the dopa decarboxylase transcript.

Additional Information

Copyright © 1981 by the American Society for Microbiology. Received 23 February 1981/Accepted 30 March 1981 We thank Ross Hodgetts for providing us with the DDC purification protocol prior to publication, Herschel Mitchell for helpful discussions, Y. H. Chien for atance in preparation and blotting of the developmental RNA preparations, Doug Barker for assistance in DNA blotting, John Frelinger for gifts of Staphylococcus protein A ghosts, and Arg Efstratiadis for helpful comments on the manuscript. This work was supported by Public Health Service research grants from the National Institutes of Health to ND. and J.H. and by a Public Health Service research fellowship from the National Institutes of Health to J.H. J.H. is presently a Mellon Scholar of the Laboratory of Human Reproduction and Reproduction Biology, Harvard Medical School.

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