Efficiency of polymerase chain reaction processes: A stochastic model

Abstract

A stochastic model for the efficiency of polymerase chain reaction (PCR) processes is presented. The model is based on the assumption that the i'th nucleotide incorporation on the DNA template takes τi seconds, where τi has an exponential distribution with mean 1/λi. In this paper, given λi that can be obtained from the primer-template sequence, temperature profile, enzyme rate, and other assay conditions [3], we calculate the efficiency of a multi-step PCR process using the distribution of the summation of non-identical exponential distributions.

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