Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published July 11, 1985 | Published
Journal Article Open

Molecular recognition of B-DNA by Hoechst 33258

Abstract

The binding sites of Hoechst 33258, netropsin and distamycin on three DNA restriction fragments from plasmid pBR322 were compared by footprinting with methidiumpropyl EDTA·Fe(II) [MPE·Fe(II)]. Hoechst, netropsin and distamycin share common binding sites that are five ± one bp in size and rich in A·T DNA base pairs. The five base pair protection patterns for Hoechst may result from a central three base pair recognition site bound by two bisbenzimidazole NHs forming a bridge on the floor of the minor groove between adjacent adenine N3 and thymine O2 atoms on opposite helix strands. Hydrophobic interaction of the flanking phenol and N-methylpiperazine rings would afford a steric blockade of one additional base pair on each side.

Additional Information

© 1985 Oxford University Press. Received 18 March 1985; Revised and Accepted 27 May 1985. Supported by the American Cancer Society research grant Number NP-428.

Attached Files

Published - HARnar85.pdf

Files

HARnar85.pdf
Files (1.9 MB)
Name Size Download all
md5:38cf08f8fc994ac4307f02473a438860
1.9 MB Preview Download

Additional details

Created:
August 22, 2023
Modified:
October 13, 2023