Response of Xenopus Cds1 in Cell-free Extracts to DNA Templates with Double-stranded Ends
- Creators
- Guo, Zijian
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Dunphy, William G.
Abstract
Although homologues of the yeast checkpoint kinases Cds1 and Chk1 have been identified in various systems, the respective roles of these kinases in the responses to damaged and/or unreplicated DNA in vertebrates have not been delineated precisely. Likewise, it is largely unknown how damaged DNA and unreplicated DNA trigger the pathways that contain these effector kinases. We report that Xenopus Cds1 (Xcds1) is phosphorylated and activated by the presence of some simple DNA molecules with double-stranded ends in cell-free Xenopus egg extracts. Xcds1 is not affected by aphidicolin, an agent that induces DNA replication blocks. In contrast, Xenopus Chk1 (Xchk1) responds to DNA replication blocks but not to the presence of double-stranded DNA ends. Immunodepletion of Xcds1 (and/or Xchk1) from egg extracts did not attenuate the cell cycle delay induced by double-stranded DNA ends. These results imply that the cell cycle delay triggered by double-stranded DNA ends either does not involve Xcds1 or uses a factor(s) that can act redundantly with Xcds1.
Additional Information
Copyright © 2000 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons–Noncommercial–Share Alike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0). Submitted November 16, 1999; Revised January 27, 2000; Accepted February 23, 2000. Monitoring Editor: Tim Hunt We thank members of the Dunphy laboratory for suggestions and comments on the manuscript. We are grateful to Yuling Sheng for technical assistance. We thank A. Kumagai for providing anti-Xchk1 antibodies and K.H. Emami, S.X. Wang, and Y. Li for supplying reagents. This work was supported in part by a grant from the National Institutes of Health. Z.G. is an associate and W.G.D. is an investigator of the Howard Hughes Medical Institute.Attached Files
Published - GUOmbc00.pdf
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Additional details
- PMCID
- PMC14865
- Eprint ID
- 4095
- Resolver ID
- CaltechAUTHORS:GUOmbc00
- NIH
- Howard Hughes Medical Institute (HHMI)
- Created
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2006-07-26Created from EPrint's datestamp field
- Updated
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2020-03-09Created from EPrint's last_modified field