Published December 1967
| Published
Journal Article
Open
Enzymatic synthesis of DNA, XXIV. Synthesis of infectious phage phiX174 DNA
Abstract
Past attempts at in vitro replication of transforming factor present in DNA have given negative or inconclusive results.(1-3) Rigid proof was lacking that template material had been excluded from the synthetic product. Even if a rigorous demonstration of net synthesis of transforming factor for a given genetic marker were forthcoming, it would still prove only that some relatively short sequence of nucleotides, sufficient for replacement of the mutant locus, had been synthesized. If enzymatic synthesis of infectious bacteriophage DNA were achieved, it would be made clear at once that relatively few, if any, mistakes had been made in replicating a DNA sequence of several thousand nucleotides.
Additional Information
© 1967 by the National Academy of Sciences. Communicated September 25, 1967. We gratefully acknowledge the expert assistance of Mrs. Gloria Davis of the Division of Biology at the California Institute of Technology in performing the spheroplast assays for infectivity. This research was supported by grants from the National Institutes of Health and the National Science Foundation. [M.G. was an] USPHS special fellow.Attached Files
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Additional details
- PMCID
- PMC223838
- Eprint ID
- 8973
- Resolver ID
- CaltechAUTHORS:GOUpnas67
- NIH
- NSF
- U.S. Public Health Service (USPHS)
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2007-10-11Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field