Structure of transcriptionally active chromatin
Abstract
Rat-liver chromatin has been fractionated into transcriptionally active and inactive regions [Gottesfeld et al. (1974) Proc. Nat. Acad. Sci. USA 71, 2193-2197] and the distribution of nuclease-resistant complexes in these fractions has been investigated. About half of the DNA of both fractions is resistant to attack by the endonuclease DNase II. The nuclease-resistant structures of inactive chromatin are DNA-histone complexes (v-bodies) which sediment at 11-13 S. Template-active chromatin yields two peaks of nuclease-resistant nucleoprotein. These complexes sediment at 14 and 19 S, and contain DNA, RNA, histone, and nonhistone chromosomal proteins. Polyacrylamide gel electrophoresis reveals a complex pattern of chromatin proteins, suggesting that the complexes are heterogeneous in composition.
Additional Information
Copyright © 1975 by the National Academy of Sciences. Contributed by James Bonner, September 10, 1975. We wish to thank Dr. K. Peters and Mr. W. Wheatley for helpful discussions. This work was supported by the U.S. Public Health Service (GM 86 and GM 13762).Files
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Additional details
- Eprint ID
- 4732
- Resolver ID
- CaltechAUTHORS:GOTpnas75
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2006-09-05Created from EPrint's datestamp field
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2019-10-02Created from EPrint's last_modified field