Published April 1981
| public
Journal Article
Open
Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase
- Creators
- Goff, Stephen
- Traktman, Paula
-
Baltimore, David
Abstract
A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Moloney murine leukemia virus have been isolated which (i) produce a thermolabile reverse transcriptase, (ii) are temperature sensitive for release of enzyme activity, or (iii) can only productively infect cells already producing gag-related polypeptides. The assay has also been useful for the isolation of nonproducer cells infected with various replication-defective transforming viruses.
Additional Information
Copyright © 1981 by the American Society for Microbiology. Received 25 November 1980. Accepted 7 January 1981. We thank Owen Witte for helpful discussions. This work was supported by contract N01-CP-53562 from the Division of Cancer Cause and Prevention, National Cancer Institute, and by Public Health Service grant CA-14051 (core grant to S.E. Luria) from the National Cancer Institute. S.P.G. is a Jane Coffin Childs Memorial Fund Fellow. D.B. is an American Cancer Society Research Professor.Files
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Additional details
- Eprint ID
- 3623
- Resolver ID
- CaltechAUTHORS:GOFjvir81a
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2006-06-22Created from EPrint's datestamp field
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2019-10-02Created from EPrint's last_modified field