Published March 1977
| Published
Journal Article
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Studies on Nucleic Acid Reassociation Kinetics: Rate of Hybridization of Excess RNA with DNA, Compared to the Rate of DNA Renaturation
Abstract
The rate of reaction of double-stranded replicative form (RF)[3H]DNA of bacteriophage phi X174 with excess (+)strand DNA and (+)strand RNA was measured by standard methods of hydroxyapatite chromatography. The reactions follow pseudo-first-order kinetics and the observed rate constant for the RNA· DNA reaction differs less than 25% from that of the DNA· DNA reaction. The pseudo-first-order rate constants are close to the value predicted on the basis of the second-order rate constant measured in the renaturation of the double-stranded phi X RF [3H]DNA.
Additional Information
Copyright © 1977 by the National Academy of Sciences Contributed by Roy J. Britten, January 10, 1977 We gratefully acknowledge the gifts of φX174 nucleic acids by Drs. Lloyd Smith, Amy S. Lee, Paul H. Johnson, and Robert L. Sinsheimer. We thank Dr. Norman Davidson for his critical review of the manuscript. This research was supported by National Institutes of Health Grants HD-05753 and GM-20927 and by National Science Foundation Grant BMS 75-07359. This is paper III in a series. Paper II is ref. 15.Attached Files
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Additional details
- PMCID
- PMC430570
- Eprint ID
- 4906
- Resolver ID
- CaltechAUTHORS:GALpnas77a
- HD-05753
- NIH
- GM-20927
- NIH
- BMS 75-07359
- NSF
- Created
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2006-09-12Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field