Monoclonal Antibodies against the Drosophila Nervous System

Creators: Fujita, Shinobu C.; Zipursky, Stephen L.; Benzer, Seymour; Ferrús, Alberto; Shotwell, Sandra L.

Abstract

A panel of 148 monoclonal antibodies directed against Drosophila neural antigens has been prepared by using mice immunized with homogenates of Drosophila tissue. Antibodies were screened immunohistochemically on cryostat sections of fly heads. A large diversity of staining patterns was observed. Some antigens were broadly distributed among tissues; others were highly specific to nerve fibers, neuropil, muscle, the tracheal system, cell nuclei, photoreceptors, or other structures. The antigens for many of the antibodies have been identified on immunoblots. Monoclonal antibodies that identify specific molecules within the nervous system should prove useful in the study of the molecular genetics of neural development.

Additional Information

© 1982 by the National Academy of Sciences. Contributed by Seymour Benzer, September 28, 1982. We thank Marika Szalay and Devra Spurr for skillful technical assistance, David Levy for introduction to hybridoma techniques, and Jeremy Brockes, Katherine Stygall, David Balzer, Gregg Lemke, Mark Tanouye, Obaid Siddiqi, Tadmiri Venkatesh, David Teplow, and Lawrence Kauvar for help and discussion. This work was supported by Grant PCM 79-11771 from the National Science Foundation and by fellowships from the Gosney (A.F.), Lawrence E. Hanson (S.L.S.), and Helen Hay Whitney (S.L.Z.) Foundations. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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