Ornithine-catalyzed urea formation in liver homogenate
- Creators
- Borsook, Henry
- Dubnoff, Jacob W.
Abstract
Heretofore attempts to obtain synthesis of urea from ammonia (and carbon dioxide) in cell-free extracts have been unsuccessful. We have found the reaction to proceed in guinea pig liver homogenate. The following is the reaction mixture which has given the highest yields obtained so far: L-ornithine (0.00075 M), ammonia (0.0025 M), L-glutamate (0.01 M), oxalacetate (0.005 M), ATP (0.00025 M), and 0.33 gm. of homogenized liver in a final volume of 3.5 ml. The following are typical increases in urea over the blank observed in 1 hour, in micrograms: ornithine + ammonia 0, glutamate + oxalacetate 0, glutamate + oxalacetate + ornithine 49, glutamate + oxalacetate + ammonia 105, glutamate + oxalacetate + ornithine + ammonia 317, glutamate + ocalacetate + ornithine + ammonia (without ATP) 150.
Additional Information
© 1947 American Society of Biological Chemists. Received for publication, May 5, 1947. This work is a part of that done under contract with the Office of Naval Research, United States Navy Department.Attached Files
Published - BORjbc47c.pdf
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Additional details
- Eprint ID
- 11849
- Resolver ID
- CaltechAUTHORS:BORjbc47c
- Office of Naval Research
- Created
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2008-10-09Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field