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Published October 28, 2003 | Published
Journal Article Open

DNA-mediated charge transport for DNA repair

Abstract

MutY, like many DNA base excision repair enzymes, contains a [4Fe4S](2+) cluster of undetermined function. Electrochemical studies of MutY bound to a DNA-modified gold electrode demonstrate that the [4Fe4S] cluster of MutY can be accessed in a DNA-mediated redox reaction. Although not detectable without DNA, the redox potential of DNA-bound MutY is approximate to275 mV versus NHE, which is characteristic of HiPiP iron proteins. Binding to DNA is thus associated with a change in [4Fe4S](3+/2+) potential, activating the cluster toward oxidation. Given that DNA charge transport chemistry is exquisitely sensitive to perturbations in base pair structure, such as mismatches, we propose that this redox process of MutY bound to DNA exploits DNA charge transport and provides a DNA signaling mechanism to scan for mismatches and lesions in vivo.

Additional Information

© 2003 by the National Academy of Sciences. Contributed by Jacqueline K. Barton, August 15, 2003. Published online before print October 14, 2003. We thank D. Ceres for technical assistance, M.-P. Golinelli for preparation of MutY mutants, and M. G. Hill and E. D. A. Stemp for helpful discussions. We thank the National Institutes of Health and the National Foundation for Cancer Research for their financial support of this research.

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