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Published January 1975 | Published
Journal Article Open

Recombinant DNA Molecules of Bacteriophage phi X174

Abstract

phi X174 DNA structures containing two different parental genomes were detected genetically and examined by electron microscopy. These structures consisted of two monomeric double-stranded DNA molecules linked in a figure 8 configuration. Such DNA structures were observed to be formed preferentially in host recA+ cells or recA+ cell-free systems. Since the host recA+ allele is required for most phi X174 recombinant formation, we conclude that the observed figure 8 molecules are intermediates in, or end products of, a phi X174 recombination event. We propose that recombinant figure 8 DNA molecules arise as a result of "single-strand aggression," are stabilized by double-strand "branch migration," and represent a specific example of a common intermediate in genetic recombination.

Additional Information

Copyright © 1975 by the National Academy of Sciences Contributed by Robert L. Sinsheimer, March 16, 1974 An oral account of this work was given to the Genetic Recombination Symposium, Bethesda, Md., 6-9 June, 1972, to the British Genetical Society, Kent, April 1973, and to the European Molecular Biology Organization meeting at Aviemore, England, June 1973. This research was supported in part by Grant GM 13554 from the U.S. Public Health Service.

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August 22, 2023
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