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Published April 1974 | Published
Journal Article Open

Genetic Recombination in Bacteriophage {varphi}X174

Abstract

Genetic recombination in bacteriophage {varphi}X174 usually takes place early in the infection process and involves two parental replicative form (double-stranded) DNA molecules. The host recA protein is required; none of the nine known {varphi}X174 cistron products is essential. The products of a single recombination event are nonreciprocal and asymmetric. Typically, only one of the parental genotypes and one recombinant genotype are recovered from a single cell. An alternative, less efficient recombination mechanism which requires an active {varphi}X174 cistron A protein is observed in the absence of the host recA gene product.

Additional Information

© 1974 American Society for Microbiology. Received for publication 25 October 1973 For the initial idea to study genetic recombination in phiX174 we are grateful to Paul Howard-Flanders. We have appreciated several exchanges regarding small phage genetic recombination with Ethel and Irwin Tessman. The excellent technical assistance of Jill Fabricant Hiatt and Alma J. Shafer is acknowledged. A preliminary account of these results was given at the Bacteriophage Meetings, Cold Spring Harbor, 26 to 31 August, 1971. This research was carried out with the assistance of Public Health Service training grant GM-86 and research grant GM 13554 from the National Institute of General Medical Sciences.

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