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Published October 1, 2004 | Published
Journal Article Open

Polarization-resolved second-harmonic-generation optical coherence tomography in collagen

Abstract

We describe a novel imaging technique, second-harmonic-generation optical coherence tomography (SHOCT). This technique combines the spatial resolution and depth penetration of optical coherence tomography (OCT) with the molecular sensitivity of second-harmonic-generation spectroscopy. As a consequence of the coherent detection required for OCT, polarization-resolved images arise naturally. We demonstrate this new technique on a skin sample from the belly of Icelandic salmon, acquiring polarization-resolved SHOCT and OCT images simultaneously.

Additional Information

© 2004 Optical Society of America Received April 27, 2004 We acknowledge funding of this work through grant R24 EB000243 from the National Institutes of Health. B. E. Applegate acknowledges fellowship support through the National Institutes of Health (T32 EB001630).

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