Continuing Rearrangement of Immunoglobulin and T-Cell Receptor Genes in a Ha-Ras-Transformed Lymphoid Progenitor Cell Line
Abstract
The arrangement of immunoglobulin genes has been examined in a series of lymphoid cell lines transformed with the Harvey murine sarcoma virus. One cell line, HAFTL-1, expresses antigenic markers characteristic of B-lymphoid cells and undergoes frequent rearrangement at the JH locus (where J = joining and H = heavy chain) during propagation in culture. By molecular cloning and nucleotide sequence determination, these rearrangements were found to represent the earliest postulated step in heavy chain gene assembly: the joining of a diversity (D) segment to a JH segment. The HAFTL-1 cell line also undergoes infrequent Dβ-to-Jβ joining at the T-cell receptor β locus in culture. The observations presented here suggest that the HAFTL-1 cell line represents the early stage of B-cell differentiation at which immunoglobulin gene rearrangement is initiated.
Additional Information
© 1987 by the National Academy of Sciences Contributed by David Baltimore, November 19, 1986 We thank Karen Wolff for excellent technical assistance. A.A. is supported by National Institutes of Health Training Grant 2 T32 CA09139. S.V.D. is an Assistant Investigator of the Howard Hughes Medical Institute. The work was supported by the Howard Hughes Medical Institute and by National Cancer Institute Grant 5 P01 CA16519 (to S.V.D.) and American Cancer Society Grant IM-355 (to D.B.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
Published - ALEpnas87.pdf
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Additional details
- PMCID
- PMC304528
- Eprint ID
- 7049
- Resolver ID
- CaltechAUTHORS:ALEpnas87
- 2 T32 CA09139
- NIH Predoctoral Fellowship
- Howard Hughes Medical Institute (HHMI)
- 5 P01 CA16519
- NIH
- IM-355
- American Cancer Society
- Created
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2007-01-06Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field