A multiwavelength digital holographic microscope architecture for enhancing life detection
Abstract
Spectral properties of living organism can provide a discriminating method for characterization. However, digital holographic microscopy (DHM), a high-resolution, volumetric imaging technique that is well-suited for examining microbial species is typically monochromatic. Thus, the spectral properties of bacteria are not probed with DHM save only for the wavelength of operation. Here we describe a new DHM instrument which enhances the method via the simultaneous recording of wavelength multiplexed holograms of the sample. This coarse spectral encoding is akin to labeling with RGB values, thereby producing colorized images of the species which enables a new dimension of characterization. This new instrument is derived from a previous architecture which has already demonstrated superior opto/mechanical stability. Herein, this novel instrument is described, and the results of experimental measurements taken with this new instrument are shown.
Additional Information
© 2019 IEEE. This work was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under contract with the National Aeronautics and Space Administration.Additional details
- Eprint ID
- 97042
- DOI
- 10.1109/AERO.2019.8742091
- Resolver ID
- CaltechAUTHORS:20190710-155312746
- NASA/JPL/Caltech
- Created
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2019-07-10Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field
- Caltech groups
- GALCIT