A method for transcriptome-wide gene expression quantification in intact tissues

Abstract

The effects of intercellular communication of immune cell types in tissues are extremely important. To screen which regulatory pathways are affected by cell–cell interactions has been very challenging, a new technology published in the March issue of Nature by Eng et al. points toward a scalable solution.1 The Improved sequential fluorescence in situ hybridization (SeqFISH+) method allows quantitative gene expression measurements of 10 000 genes in situ within intact tissue samples at single‐cell resolution, within thousands of single cells.

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