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Published June 2001 | public
Journal Article

Inhibition of Gene Expression by Homologous Double-Stranded RNA in a Drosophila melanogaster Cell Culture

Abstract

Specific inhibition of gene expression by exogenous homologous double-stranded RNA (dsRNA) in invertebrates and in the early development of vertebrates is termed RNA interference. Cultured cells were cotransfected with reporter plasmids and dsRNA. The inhibitory effect on reporter gene expression depended on the extent of homology between dsRNA and the target gene. RNA interference was also studied in cells cotransfected with plasmids directing synthesis of sense and antisense RNAs. Production of antisense RNA only slightly inhibited expression of the reporter gene. Simultaneous expression of both sense and antisense RNAs caused by cotransfection by corresponding plasmids did not inhibit expression of the reporter construct.

Additional Information

© 2001 MAIK "Nauka/Interperiodica". Russian Journal of Genetics, Vol. 37, No. 6, 2001, pp. 639–642. Translated from Genetika, Vol. 37, No. 6, 2001, pp. 779–783. Original Russian Text Copyright © 2001 by Aravin, Vagin, Rozovsky, Gvozdev. Received January 3, 2001. This work was supported by the Russian Foundation for Basic Research (project nos. 99-04-48561, 00-15-97896) and the Russian programs "Frontiers in Genetics" (project no. 99-1069) and "Russian Universities: Basic Research" (project no. 5244).

Additional details

Created:
August 21, 2023
Modified:
October 20, 2023