Analysis of Large-Scale Sequencing of Small RNAs
- Other:
- Altman, Russ
Abstract
The advent of large-scale sequencing has opened up new areas of research, such as the study of Piwi-interacting small RNAs (piRNAs). piRNAs are longer than miRNAs, close to 30 nucleotides in length, involved in various functions, such as the suppression of transposons in germline 3,4,5. Since a large number of them (many tens of thousands) are generated from a wide range of positions in the genome, large-scale sequencing is the only way to study them. The key to understanding their genesis and biological roles is efficient analysis, which is complicated by the large volumes of sequence data. Taking account of the underlying biology is also important. We describe here novel analyses techniques and tools applied to small RNAs from germ cells in D. melanogaster, that allowed us to infer mechanism and biological function.
Additional Information
© 2008 World Scientific Publishing Company. The authors acknowledge the help of Ted Roeder and Ankit Patel with various aspects of the front end for the web-based software and the anonymous reviewers for suggesting numerous improvements to the manuscript.Additional details
- Eprint ID
- 95361
- DOI
- 10.1142/9789812776136_0014
- Resolver ID
- CaltechAUTHORS:20190509-081714112
- Created
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2019-05-09Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field