Published May 2019
| Supplemental Material
Journal Article
Open
Phase separation drives X-chromosome inactivation: a hypothesis
Abstract
The long non-coding RNA Xist induces heterochromatinization of the X chromosome by recruiting repressive protein complexes to chromatin. Here we gather evidence, from the literature and from computational analyses, showing that Xist assemblies are similar in size, shape and composition to phase-separated condensates, such as paraspeckles and stress granules. Given the progressive sequestration of Xist's binding partners during X-chromosome inactivation, we formulate the hypothesis that Xist uses phase separation to perform its function.
Additional Information
© 2019 Nature Publishing Group. Published 06 May 2019; Issue Date May 2019. We thank all members of the Avner, Tartaglia and Guttman groups, as well as Greta Pintacuda and Kathrin Plath for critical reading of the manuscript. P.A. was funded by an EMBL grant (50800), and A.C. was funded by an EMBL fellowship and a Rett Syndrome Research Trust (RSRT) grant. The research leading to these results has been supported by the European Research Council (RIBOMYLOME_309545), the Spanish Ministry of Economy, Industry, and Competitiveness (BFU2014-55054-P and BFU2017-86970-P) and "Fundació La Marató de TV3" (PI043296). M.G. was funded by a Caltech grant. We acknowledge support from the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) to the EMBL partnership, the Centro de Excelencia Severo Ochoa and the CERCA Programme/Generalitat de Catalunya.Attached Files
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Additional details
- Eprint ID
- 95344
- DOI
- 10.1038/s41594-019-0223-0
- Resolver ID
- CaltechAUTHORS:20190508-095119340
- 50800
- European Molecular Biology Laboratory (EMBL)
- Rett Syndrome Research Trust
- RIBOMYLOME_309545
- European Research Council (ERC)
- BFU2014-55054-P
- Ministerio de Economía, Industria y Competitividad (MINECO)
- BFU2017-86970-P
- Ministerio de Economía, Industria y Competitividad (MINECO)
- PI043296
- Fundació La Marató de TV3
- Caltech
- Centro de Excelencia Severo Ochoa
- Generalitat de Catalunya
- Created
-
2019-05-08Created from EPrint's datestamp field
- Updated
-
2023-10-20Created from EPrint's last_modified field