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Published August 2019 | Accepted Version + Submitted + Supplemental Material
Journal Article Open

A novel uncultured marine cyanophage lineage with lysogenic potential linked to a putative marine Synechococcus 'relic' prophage

Abstract

Marine cyanobacteria are important contributors to primary production in the ocean and their viruses (cyanophages) affect the ocean microbial communities. Despite reports of lysogeny in marine cyanobacteria, a genome sequence of such temperate cyanophages remains unknown although genomic analysis indicate potential for lysogeny in certain marine cyanophages. Using assemblies from Red Sea and Tara Oceans metagenomes, we recovered genomes of a novel uncultured marine cyanophage lineage, which contain, in addition to common cyanophage genes, a phycobilisome degradation protein NblA, an integrase and a split DNA polymerase. The DNA polymerase forms a monophyletic clade with a DNA polymerase from a genomic island in Synechococcus WH8016. The island contains a relic prophage that does not resemble any previously reported cyanophage but shares several genes with the newly identified cyanophages reported here. Metagenomic recruitment indicates that the novel cyanophages are widespread, albeit at low abundance. Here, we describe a novel potentially lysogenic cyanophage family, their abundance and distribution in the marine environment.

Additional Information

© 2019 Wiley-Blackwell. Issue Online: 02 July 2019; Version of Record online: 17 June 2019; Accepted manuscript online: 24 May 2019; Manuscript accepted: 23 May 2019; Manuscript received: 21 October 2018. Author Contributions: J.F.-U. and O.B conceived the project. J.F.-U., A.P., I.S., and O.B. performed bioinformatic analyses. J.F.-U., S.F. and S.L. performed mitomycin C experiments. J.F.-U. and O.B. wrote the manuscript with contributions from all authors to data analysis, figure generation, and the final manuscript. We thank Laurence Garczarek for sharing unpublished data for Synechococcus WH8016, and Curtis Suttle and Caroline Chénard for sharing DNA polymerase sequences. This work was funded by a European Commission (ERC Advanced Grant no. 321647), and the Louis and Lyra Richmond Memorial Chair in Life Sciences (to O.B.). The authors declare no conflict of interest.

Attached Files

Accepted Version - 1758-2229.12773.pdf

Submitted - 325100.full.pdf

Supplemental Material - emi412773-sup-0001-supinfo01.txt

Supplemental Material - emi412773-sup-0002-supinfo02.xls

Supplemental Material - emi412773-sup-0003-supinfo03.txt

Supplemental Material - emi412773-sup-0004-supinfo04.txt

Supplemental Material - emi412773-sup-0005-supinfo05.xls

Supplemental Material - emi412773-sup-0006-supinfo06.pdf

Supplemental Material - emi412773-sup-0007-supinfo07.xls

Supplemental Material - emi412773-sup-0008-supinfo08.xlsx

Supplemental Material - emi412773-sup-0009-supinfo09.txt

Supplemental Material - emi412773-sup-0010-supinfo10.txt

Supplemental Material - emi412773-sup-0011-supinfo11.txt

Supplemental Material - emi412773-sup-0012-supinfo12.docx

Supplemental Material - emi412773-sup-0013-figures1.eps

Supplemental Material - emi412773-sup-0014-figures2.eps

Supplemental Material - emi412773-sup-0015-figures3.pdf

Supplemental Material - emi412773-sup-0016-figures4.pdf

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Additional details

Created:
August 19, 2023
Modified:
October 20, 2023