Structured illumination imaging without grating rotation based on mirror operation on 1D Fourier spectrum
Abstract
Structured illumination microscopy (SIM) is a rapidly developing a super-resolution optical microscopy technique. With SIM, the grating is needed in order to rotate several angles for illuminating the sample in different directions. Multiple rotations reduce the imaging speed and grating rotation angle errors damage the image recovery quality. We introduce mirror transformation on one-dimension (1D) Fourier spectrum to SIM for resolving the problems of low imaging speed and severe impact on image reconstruction quality by grating rotation angle errors. When mirror operation and SIM are combined, the grating is placed at an orientation for obtaining three shadow images. The three shadow images are acquired by CCD at three different phase shift for a direction of grating. Thus, the SIM imaging speed is faster and the effect on image reconstruction quality by grating rotation angle errors is greatly reduced.
Additional Information
© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. Received 22 Nov 2018; revised 14 Jan 2019; accepted 15 Jan 2019; published 23 Jan 2019. Funding: National Natural Science Foundation of China (Nos. 61575055, 61575053, 11874132); National Institutes of Health (NIH) (Grant ROI EY024628); and Open Fund Project Foundation of Guangdong Provincial Key Laboratory of Modern Geometric and Mechanical Metrology Technology (No. SCMKF201804). The authors express sincere gratitude to Dr. Benquan Wang for his assistance in experimental data.Attached Files
Published - oe-27-3-2016.pdf
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Additional details
- PMCID
- PMC6410912
- Eprint ID
- 93015
- Resolver ID
- CaltechAUTHORS:20190221-075930981
- 61575055
- National Natural Science Foundation of China
- 61575053
- National Natural Science Foundation of China
- 11874132
- National Natural Science Foundation of China
- R01 EY024628
- NIH
- SCMKF201804
- Foundation of Guangdong Provincial Key Laboratory of Modern Geometric and Mechanical Metrology Technology
- Created
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2019-02-21Created from EPrint's datestamp field
- Updated
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2023-06-06Created from EPrint's last_modified field