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Published June 17, 1999 | Published
Book Section - Chapter Open

Scanning apertureless fluorescence microscope

Abstract

We describe a near-field apertureless fluorescence microscope, capable of imaging fluorescent latex beads with subwavelength precision. The instrument is based on a home- built tapping-mode atomic-force microscope, to which an inverted optical microscope was added. The fact that the wavelength of the fluorescence that we observe is different from the wavelength of the illumination allows for a relatively straightforward detection mechanism. Sample images are presented, along with evidence that the observe effect is of optical origin.

Additional Information

© 1999 Society of Photo-optical Instrumentation Engineers (SPIE). This work was supported by the Keck Foundation. G. L. had partial financial support from the Fonds FCAR.

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