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Published October 15, 1996 | Published
Journal Article Open

Adenovirus-mediated gene delivery into neuronal precursors of the adult mouse brain

Abstract

Precursor cells found in the subventricular zone (SVZ) of the adult brain can undergo cell division and migrate long distances before differentiating into mature neurons. We have investigated the possibility of introducing genes stably into this population of cells. Replication-defective adenoviruses were injected into the SVZ of the lateral ventricle of adult mice. The adenoviruses carried a cDNA for the LacZ reporter or the human p75 neurotrophin receptor, for which species-specific antibodies are available. Injection of the viruses into the SVZ led to efficient labeling of neuronal precursors. Two months after viral injection, infected cells were detected in the olfactory bulb, a significant distance from the site of injection. Labeled periglomerular and granular neurons with extensive dendritic arborization were found in the olfactory bulb. These results demonstrate that foreign genes can be efficiently introduced into neuronal precursor cells. Furthermore, adenovirus-directed infection can lead to long-term stable gene expression in progenitor cells found in the adult central nervous system.

Additional Information

© 1996 National Academy of Sciences. Communicated by Kenneth I. Berns, Cornell University Medical College, New York, NY, July 31, 1996 (received for review February 7, 1996) We thank B. Bewig and R. Crystal for the LacZ adenovirus. This project was supported by a grant to S.O.Y. by the Multiple Sclerosis Society, grants to M.V.C. from the National Institutes of Health and the Dorothy Rodbell Cohen Foundation, and grants from National Institutes of Health (Program Project HL 51746 to E.F.-P. and HD 32116 to A.A.-B.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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August 19, 2023
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