Distinct Mammalian Precursors Are Committed to Generate Neurons with Defined Dendritic Projection Patterns
Abstract
The mechanisms that regulate how dendrites target different neurons to establish connections with specific cell types remain largely unknown. In particular, the formation of cell-type–specific connectivity during postnatal neurogenesis could be either determined by the local environment of the mature neuronal circuit or by cell-autonomous properties of the immature neurons, already determined by their precursors. Using retroviral fate mapping, we studied the lamina-specific dendritic targeting of one neuronal type as defined by its morphology and intrinsic somatic electrical properties in neonatal and adult neurogenesis. Fate mapping revealed the existence of two separate populations of neuronal precursors that gave rise to the same neuronal type with two distinct patterns of dendritic targeting—innervating either a deep or superficial lamina, where they connect to different types of principal neurons. Furthermore, heterochronic and heterotopic transplantation demonstrated that these precursors were largely restricted to generate neurons with a predetermined pattern of dendritic targeting that was independent of the host environment. Our results demonstrate that, at least in the neonatal and adult mammalian brain, the pattern of dendritic targeting of a given neuron is a cell-autonomous property of their precursors.
Additional Information
© 2007 Kelsch et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The project was funded by a grant from the David and Lucille Packard Foundation to CL. Grant support: M.I.T. Newton Fellowship to WK, Singleton M.I.T. Fellowship to CWL, and David and Lucille Packard Foundation to CL. We thank D. Friedmann, E. Hong, and S. Magavi for critically reading the manuscript; S. Song for valuable suggestions regarding statistical analysis; and V. Egger for initial help with the preparation of acute olfactory bulb slices. Author Contributions: WK and CL conceived and designed the experiments and wrote the paper. WK, CPM, and CWL performed the experiments and analyzed the data. WK and CL contributed reagents/materials/analysis tools. The authors have declared that no competing interests exist.Errata
Kelsch W, Mosley CP, Lin CW, Lois C (2008) Correction: Distinct Mammalian Precursors Are Committed to Generate Neurons with Defined Dendritic Projection Patterns. PLOS Biology 6(4): e91. https://doi.org/10.1371/journal.pbio.0060091 Kelsch W, Mosley CP, Lin CW, Lois C (2007) Correction: Distinct Mammalian Precursors Are Committed to Generate Neurons with Defined Dendritic Projection Patterns. PLOS Biology 5(12): e336. https://doi.org/10.1371/journal.pbio.0050336Attached Files
Published - journal.pbio.0050300.PDF
Supplemental Material - journal.pbio.0050300.sd001.DOC
Supplemental Material - journal.pbio.0050300.sg001.TIF
Supplemental Material - journal.pbio.0050300.sg002.TIF
Erratum - pbio.0050336.pdf
Erratum - pbio.0060091.pdf
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Additional details
- PMCID
- PMC2071944
- Eprint ID
- 90057
- Resolver ID
- CaltechAUTHORS:20181001-113519846
- David and Lucile Packard Foundation
- Massachusetts Institute of Technology (MIT)
- Created
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2018-10-05Created from EPrint's datestamp field
- Updated
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2023-06-01Created from EPrint's last_modified field