Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published February 15, 2019 | Supplemental Material + Accepted Version + Submitted
Journal Article Open

Draxin alters laminin organization during basement membrane remodeling to control cranial neural crest EMT

Abstract

Premigratory neural crest cells arise within the dorsal neural tube and subsequently undergo an epithelial-to-mesenchymal transition (EMT) to leave the neuroepithelium and initiate migration. Draxin is a Wnt modulator that has been shown to control the timing of cranial neural crest EMT. Here we show that this process is accompanied by three stages of remodeling of the basement membrane protein laminin, from regression to expansion and channel formation. Loss of Draxin results in blocking laminin remodeling at the regression stage, whereas ectopic maintenance of Draxin blocks remodeling at the expansion stage. The latter effect is rescued by addition of Snail2, previously shown to be downstream of Draxin. Our results demonstrate an essential function for the Wnt modulator Draxin in regulating basement membrane remodeling during cranial neural crest EMT.

Additional Information

© 2018 Elsevier. Received 31 August 2018, Revised 19 December 2018, Accepted 19 December 2018, Available online 21 December 2018. We thank Dr. M. Simoes-Costa for providing the NC1.1 M3:EGFP construct, and Dr. M. Cheung for providing the pCIG-V5-Snail2-IRES-nls-GFP construct (Addgene plasmid # 44282). This work was supported by a National Institutes of Health grant (R01DE024157 and PO1HD037105 to M.E. Bronner), and a Ruth L. Kirschstein National Research Service Award (F32DE026355 to E.J. Hutchins). The authors declare no competing interests.

Attached Files

Accepted Version - 1-s2.0-S001216061830589X-main_acc.pdf

Accepted Version - nihms-1517618.pdf

Submitted - 407882.full.pdf

Supplemental Material - 1-s2.0-S001216061830589X-mmc1.pdf

Files

1-s2.0-S001216061830589X-main_acc.pdf
Files (16.4 MB)
Name Size Download all
md5:ea41b87972b10f04c438dd8ee6a06d87
4.6 MB Preview Download
md5:c65481c3c44da3b6415764feb8b53db4
5.7 MB Preview Download
md5:aa391c2d08c901d3f8cd1a404fd9a711
3.8 MB Preview Download
md5:92160e6f0671bc48b46a6d2e020c465d
2.2 MB Preview Download

Additional details

Created:
August 22, 2023
Modified:
October 23, 2023