Dye-Sensitized Core/Active Shell Upconversion Nanoparticles for Optogenetics and Bioimaging Applications
Abstract
Near-infrared (NIR) dye-sensitized upconversion nanoparticles (UCNPs) can broaden the absorption range and boost upconversion efficiency of UCNPs. Here, we achieved significantly enhanced upconversion luminescence in dye-sensitized core/active shell UCNPs via the doping of ytterbium ions (Yb^(3+)) in the UCNP shell, which bridged the energy transfer from the dye to the UCNP core. As a result, we synergized the two most practical upconversion booster effectors (dye-sensitizing and core/shell enhancement) to amplify upconversion efficiency. We demonstrated two biomedical applications using these UCNPs. By using dye-sensitized core/active shell UCNP embedded poly(methyl methacrylate) polymer implantable systems, we successfully shifted the optogenetic neuron excitation window to a biocompatible and deep tissue penetrable 800 nm wavelength. Furthermore, UCNPs were water-solubilized with Pluronic F127 with high upconversion efficiency and can be imaged in a mouse model.
Additional Information
© 2016 American Chemical Society. Received: October 10, 2015; Accepted: January 6, 2016; Published: January 6, 2016. This research was supported by the China Scholarship Council (CSC) to X.W., a Worcester Foundation Mel Cutler Award, National Institutes of Health R01MH103133 to G.H, C.L., and Y.X., Human Frontier Science Program RGY-0090/2014 to G.H. and Y.X. Quantum yield work (E.M.C.) at the Molecular Foundry was supported by the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. D.L. is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences Early Career Research Program, under Award No. 67037. We also thank Dr. Shaul Aloni's help in TEM measurements. Author Contributions: X. Wu, Y. Zhang, and K. Takle contributed equally to this work. The authors declare no competing financial interest.Attached Files
Accepted Version - nihms794148.pdf
Supplemental Material - nn5b06383_si_001.pdf
Files
Name | Size | Download all |
---|---|---|
md5:d2e5d30e1c6e1bef37914bd593fe5ade
|
597.2 kB | Preview Download |
md5:ef54d3fdf3d83d0d98a35c3bfa73e0ac
|
2.7 MB | Preview Download |
Additional details
- PMCID
- PMC4913696
- Eprint ID
- 89976
- Resolver ID
- CaltechAUTHORS:20180926-144824986
- China Scholarship Council
- Worcester Foundation
- R01MH103133
- NIH
- RGY-0090/2014
- Human Frontier Science Program
- DE-AC02-05CH11231
- Department of Energy (DOE)
- 67037
- Department of Energy (DOE)
- Created
-
2018-09-26Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field