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Published September 1990 | public
Journal Article

Engineering enzymes for non-aqueous solvents

Abstract

Enzymes may be redesigned to permit catalysis in non-aqueous solvents by engineering their amino acid sequences, thereby altering their physical and chemical properties to suit the new solvent environment. The interactions that contribute to protein stability in non-aqueous solvents are discussed in the context of attempting to identify possible approaches to constructing enzymes which exhibit enhanced stability in non-aqueous media. These approaches are illustrated by several examples where protein engineering has resulted in enzymes that are better suited for catalysis in organic solvents.

Additional Information

© 1990 Published by Elsevier Ltd.

Additional details

Created:
August 19, 2023
Modified:
October 18, 2023