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Published May 5, 1996 | public
Journal Article

Two‐dimensional electrophoresis of proteins as a tool in the metabolic engineering of cell cycle regulation

Abstract

Metabolic engineering of cell cycle regulation addresses important biotechnological problems about serum removal from animal cell culture systems. Chinese hamster ovary cells stimulated to grow by fetal calf serum, insulin, or basic fibroblast growth factor were studied by two‐dimensional electrophoresis (2DE) and the resulting protein expression patterns were analyzed. Detailed 2DE protocols are provided and at least 24 gene products are identified which may play an important role in growth factor signaling. Moreover, a correlation between the expression of three proteins (cyclin D1, cyclin E, and E2F‐1) and mitogenic strength was found.

Additional Information

© 1996 John Wiley & Sons. Received July 11, 1995; Accepted December 7, 1995. The authors thank Bengt Bjellqvist, Wolfgang Renner, Adriana Sburlati, Cornelia Schwerdel, and Christian Weikert for useful discussions and technical assistance. This work was funded by the Swiss Priority Program in Biotechnology Grant No. 5002-037031.

Additional details

Created:
August 22, 2023
Modified:
October 18, 2023