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Published March 1993 | public
Journal Article

Insect cell hosts for baculovirus expression vectors contain endogenous exoglycosidase activity

Abstract

Four different insect cell lines that can be used as hosts for baculovirus infection were assayed for the presence of endogenous exoglycosidases. All four cell lines, derived from Spodoptera frugiperda, Trichoplusia ni, Bombyx mori, or Malacosoma disstria, contained N-acetyl-β-glucosaminidase, N-acetyl-β-galactosaminidase, β-galactosidase, and sialidase activities. Exoglycosidase activities were found in cell lysates as well as cell-free supernatants from uninfected and wild-type baculovirus infected cells. Oligosaccharide analysis of cellular glycoproteins using lectins recognizing Galβ1, 3GalNAc, Galβl, 4GlcNAc, and NeuAcα2,6Gal demonstrated that only Galβl, 3GalNAc was present. The demonstration that these cells contain exoglycosidases raises the possibility that the oligosaccharides of baculovirus-expressed glycoproteins are subject to enzymatic degradation.

Additional Information

© 1993 American Chemical Society. Accepted January 7, 1993. We are grateful to Dr. Charles Goochee and Michael Gramer for their advice and critical review of this manuscript. This research was supported by the National Science Foundation (Grant No. BCS-8721973), by a grant for Predoctoral Training in Biotechnology from the National Institute of General Medical Sciences, by National Research Service Award 1 T32 GM 08346-01 from the Pharmacology Sciences Program, and by a Kelco Graduate Fellowship.

Additional details

Created:
August 20, 2023
Modified:
October 18, 2023