In vivo study of optical speckle decorrelation time across depths in the mouse brain
Abstract
The strong optical scattering of biological tissue confounds our ability to focus light deeply into the brain beyond depths of a few hundred microns. This challenge can be potentially overcome by exploiting wavefront shaping techniques which allow light to be focused through or inside scattering media. However, these techniques require the scattering medium to be static, as changes in the arrangement of the scatterers between the wavefront recording and playback steps reduce the fidelity of the focus that is formed. Furthermore, as the thickness of the scattering medium increases, the influence of the dynamic nature becomes more severe due to the growing number of scattering events experienced by each photon. In this paper, by examining the scattering dynamics in the mouse brain in vivo via multispeckle diffusing wave spectroscopy (MSDWS) using a custom fiber probe that simulates a point-like source within the brain, we investigate the relationship between this decorrelation time and the depth of the point-like light source inside the living mouse brain at depths up to 3.2 mm.
Additional Information
© 2017 Optical Society of America. Received 16 Aug 2017; revised 10 Sep 2017; accepted 25 Sep 2017; published 4 Oct 2017. Disclosures: The authors declare that there are no conflicts of interest related to this article. This work was supported in part by GIST Research Institute (GRI), and the GIST-Caltech Research Collaboration Project through a grant provided by GIST in 2017, a research grant (NRF-2016R1A2B4015381), the Brain Research Program (NRF-2017M3C7A 1044964) of the National Research Foundation (NRF), the KBRI basic research program through Korea Brain Research Institute (17-BR-04) funded by the Ministry of Science, ICT, Future Planning, the industrial convergence foundation construction program (N0002310) funded by the Ministry of Trade, Industry & Energy, and the National Institutes of Health (NIH) (U01NS090577). J.B. was supported by the National Institute of Biomedical Imaging, and Bioengineering (F31EB021153) under a Ruth L. Kirschstein National Research Service Award and by the Donna and Benjamin M. Rosen Bioengineering Center. The authors thank Ms. Ji Hye Yang from GIST for her help during the animal experiment.Errata
The last sentence of the 2nd paragraph of section "4. Results" should read: "The respective mean decorrelation times for 1.1, 1.8, 2.5, and 3.2 mm depth of point-like source are 5.3, 1.5, 0.37, and 0.26 ms, with standard deviations of 4.8, 1.6, 0.16, 0.13 ms." instead of the version in the manuscript (copied below) which contains incorrect values. "The respective mean decorrelation times for 1.1, 1.8, 2.5, and 3.2 mm depth of point-like source are 7.5, 3.5, 1.1, and 0.6 ms, with standard deviations of 5.9, 2.4, 0.6, and 0.3 ms."Attached Files
Published - boe-8-11-4855.pdf
Erratum - boe-8-11-5039_errtm.pdf
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Additional details
- PMCID
- PMC5695936
- Eprint ID
- 83283
- Resolver ID
- CaltechAUTHORS:20171117-083356029
- GIST Research Institute (GRI)
- GIST-Caltech Research Collaboration Project
- NRF-2016R1A2B4015381
- National Research Foundation of Korea
- NRF-2017M3C7A 1044964
- National Research Foundation of Korea
- 17-BR-04
- Ministry of Science, ICT, Future Planning (Korea)
- N0002310
- Ministry of Trade, Industry and Energy (Korea)
- U01NS090577
- NIH
- F31EB021153
- NIH
- Donna and Benjamin M. Rosen Bioengineering Center
- Created
-
2017-11-20Created from EPrint's datestamp field
- Updated
-
2022-03-22Created from EPrint's last_modified field
- Caltech groups
- Rosen Bioengineering Center