Published November 13, 2017
| public
Book Section - Chapter
RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells
- Creators
- McHugh, Colleen A.
- Guttman, Mitchell
- Other:
- Gaspar, Imre
Abstract
RNA molecules interact with proteins to perform a variety of functions in living cells. The binding partners of many RNAs, in particular the newly discovered class of long noncoding RNAs (lncRNAs), remain largely unknown. RNA antisense purification coupled with mass spectrometry (RAP-MS) is a method that enables the identification of direct and specific protein interaction partners of a specific RNA molecule. Because RAP-MS uses direct RNA–protein cross-linking methods coupled along with highly denaturing purification conditions, RAP-MS provides a short list of high confidence protein interactors.
Additional Information
© 2018 Springer Science+Business Media LLC. First Online: 13 November 2017.Additional details
- Eprint ID
- 83227
- Resolver ID
- CaltechAUTHORS:20171115-111045657
- Created
-
2017-11-15Created from EPrint's datestamp field
- Updated
-
2021-11-15Created from EPrint's last_modified field
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 1649