Ionic Liquid-Based Periodic Mesoporous Organosilica: An Innovative Matrix for Enzyme Immobilization

Abstract

Employment of enzyme immobilization technique for α-amylase, provides useful approaches to improve enzyme resistance to harsh conditions and reusability. Two silica supports, Ionic Liquid-Based Periodic Mesoporous Organosilica which we call it PMO-IL, and SBA-15, have been used for immobilization of α-amylase. We found that proper electrostatic interactions result in a higher immobilization yield of PMO-IL rather than SBA-15. The kinetics parameters show that α-amylase@PMO-IL activity has slightly decreased in comparison to free enzyme and the stability studies indicate that it is even more active than free enzyme in the natural pH which shows the improving role of ionic liquid on enzyme structure and function. Also, both immobilized enzymes have 2 times more stability at 70 °C and 80 °C after 60 min in comparison to free enzyme. Finally, the recovery efficiency of α-amylase@PMO-IL is 88% of its initial activity after 4 cycles which is 23% more than a previous report.

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