Published 2016
| public
Book Section - Chapter
Electron cryotomography
Abstract
Electron cryotomography (ECT) delivers 3D images of native structures inside intact cells with resolution on the scale of large macromolecular complexes (~ 4 nm). It has proven to be an invaluable tool for interrogating the organization of bacterial cells and the structures of the nanomachines inside them. Here we present a brief introduction to the technique, a few examples of what we have learned by imaging bacterial cells with ECT over the last 15 years and a frank discussion of the relative advantages and limitations of the technique compared to some other popular imaging methods used in microbiology.
Additional Information
© 2016 Elsevier Ltd. Available online 27 October 2016.Additional details
- Eprint ID
- 80808
- Resolver ID
- CaltechAUTHORS:20170825-145104411
- Created
-
2017-08-28Created from EPrint's datestamp field
- Updated
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2022-10-24Created from EPrint's last_modified field
- Series Name
- Methods in Microbiology
- Series Volume or Issue Number
- 43