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Published May 2010 | Published
Book Section - Chapter Open

Double-Helix Microscopy for Wide-Field 3-D Single-Molecule Fluorescence Imaging

Abstract

We present methods to improve the localization accuracy in wide-field 3-D single-molecule double-helix microscopy. We analyze the optical efficiency of the system, the fundamental limit for 3-D localization, the estimation algorithms, and polarization sensitive detection.

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© 2010 The Optical Society.

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