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Published March 3, 2017 | Submitted
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Near-optimal RNA-Seq quantification

Abstract

We present a novel approach to RNA-Seq quantification that is near optimal in speed and accuracy. Software implementing the approach, called kallisto, can be used to analyze 30 million unaligned paired-end RNA-Seq reads in less than 5 minutes on a standard laptop computer while providing results as accurate as those of the best existing tools. This removes a major computational bottleneck in RNA-Seq analysis.

Additional Information

Simulations and analysis: The parameters and procedures used for the results and figures in the paper are available via a snakefile16 at https://github.com/pachterlab/kallisto_paper_analysis. Software: The kallisto program is available for download from http://pachterlab.github.io/kallisto.

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August 20, 2023
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October 24, 2023