Kinetic Model for the ATP-Dependent Translocation of Saccharomyces cerevisiae RSC along Double-Stranded DNA
Abstract
The chromatin remodeling complex RSC from Saccharomyces cerevisiae is a DNA translocase that moves with directionality along double-stranded DNA in a reaction that is coupled to ATP hydrolysis. To better understand how this basic molecular motor functions, a novel method of analysis has been developed to study the kinetics of RSC translocation along double-stranded DNA. The data provided are consistent with RSC translocation occurring through a series of repeating uniform steps with an overall processivity of P = 0.949 ± 0.003; this processivity corresponds to an average translocation distance of 20 ± 1 base pairs (bp) before dissociation. Interestingly, a slow initiation process, following DNA binding, is required to make RSC competent for DNA translocation. These results are further discussed in the context of previously published studies of RSC and other DNA translocases.
Additional Information
© 2007 American Chemical Society. Received 15 May 2007. Published online 5 October 2007. Published in print 1 October 2007. This research was supported, in part, by startup funding from the University of Kansas (to C.J.F.) and by the National Institutes of Health (GM60415 to B.R.C., support of A.S.). B.R.C. is an Investigator with the Howard Hughes Medical Institute. We thank the University of Kansas Department Molecular Biosciences and especially Mark Richter for the use of common equipment. In addition, we thank the University of Kansas Department of Pharmacology and Toxicology, especially Dr. Abdulbaki Agbas and Dr. Elias K. Michaelis, for the use of the department's phosphorimager. We thank Janet Lindsley (Department of Biochemistry, University of Utah) for generously allowing access to stopped-flow equipment and for expert advice on conducting the experiments. We thank Bob Schackmann for oligonucleotide synthesis (Core Facilities).Attached Files
Accepted Version - nihms-84034.pdf
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Additional details
- PMCID
- PMC2810488
- Eprint ID
- 73905
- Resolver ID
- CaltechAUTHORS:20170201-074622359
- University of Kansas
- GM60415
- NIH
- Howard Hughes Medical Institute (HHMI)
- Created
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2017-02-01Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field