The CLAVATA1 Gene Encodes a Putative Receptor Kinase That Controls Shoot and Floral Meristem Size in Arabidopsis
Abstract
The shoot apical meristem is responsible for above-ground organ initiation in higher plants, accomplishing continuous organogenesis by maintaining a pool of undifferentiated cells and directing descendant cells toward organ formation. Normally, proliferation and differentiation are balanced, so that the structure and size of the shoot meristem is maintained. However, Arabidopsis plants homozygous for mutations at the CLAVATA1 (CLV1) locus accumulate excess undifferentiated cells. We describe the molecular cloning and expression pattern of the CLV1 gene. It encodes a putative receptor kinase, suggesting a role in signal transduction. The extracellular domain is composed of 21 tandem leucine-rich repeats that resemble leucine-rich repeats found in animal hormone receptors. We provide evidence that CLV1 expression in the inflorescence is specifically associated with meristematic activity.
Additional Information
© 1997 Cell Press. Received 27 February 1997, Revised 11 April 1997. The authors would like to thank Hajime Sakai and Leonard Medrano for invaluable technical assistance and advice. We also thank Ephraim Simon for sectioning material for in situ hybridization, Amy Trotochaud for technical assistance in generating transgenic plants, and Michael Frohlich for assistance with the phylogenetic analysis. We are grateful to Steve Jacobsen for isolating the clv1–9 allele and June Medford for providing the clv1–8 allele. We also thank X. Chen, J. Fletcher, J. Hua, J.-L. Riechmann, M. Running, R. Sablowski, H. Sakai, D. Wagner, and E. Zeigelhoffer for careful review of this manuscript. ga2 seeds were provided by the Arabidopsis Biological Resource Center at Ohio State University. This work was supported by NSF grant MCB-9204839 and a Strategic Research Fund Grant from Zeneca Seeds to E. M. M., and an NSF Developmental Mechanisms grant to S. E. C. (IBN 9506952). S. E. C. was an NSF Plant Biology Postdoctoral Fellow, and R. W. W. was supported by NIH Predoctoral Training Grant GM07616 and the Howard Hughes Medical Institute. The GenBank accession numbers for the sequences reported in this article are U96879 for CLV1 and U96877 and U96878 for AtVPS-35.Additional details
- Eprint ID
- 72836
- Resolver ID
- CaltechAUTHORS:20161214-155104303
- MCB-9204839
- NSF
- Zeneca Seeds
- IBN-9506952
- NSF
- NSF Plant Biology Postdoctoral Fellowship
- GM07616
- NIH Predoctoral Fellowship
- Howard Hughes Medical Institute (HHMI)
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2016-12-15Created from EPrint's datestamp field
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2021-11-11Created from EPrint's last_modified field