A Homolog of NO APICAL MERISTEM Is an Immediate Target of the Floral Homeotic Genes APETALA3/PISTILLATA
Abstract
To understand how homeotic genes affect morphogenesis and differentiation, their target genes must be identified. In Arabidopsis flowers, the homeotic protein heterodimer APETALA3/PISTILLATA is necessary for petal and stamen formation. Here, AP3/PI function was put under posttranslational control to analyze its immediate effect on the floral mRNA population, with indirect effects blocked by cycloheximide. Using differential display, a target gene of AP3/PI was identified (NAP: NAC-LIKE, ACTIVATED BY AP3/PI), which is homologous to genes required for meristem establishment and separation of floral organs. The expression pattern of NAP and the phenotypes caused by its misexpression suggest that it functions in the transition between growth by cell division and cell expansion in stamens and petals.
Additional Information
© 1998 Cell Press. Received 3 November 1997, Revised 24 November 1997. We are grateful to L. Medrano for help with restriction fragment length polymorphism mapping, T. Jack for pF730, B. Krizek for the 35S::PI plasmid, A. Lloyd for pBI-ΔGR, J. L. Riechmann for PromoterFinder libraries, M. Callahan for help with PCR genotyping, P. Koen for help with scanning electron microscopy, and to K. Baker, X. Chen, C. -F. Chuang, J. Fletcher, J. Hua, T. Ito, C. Ohno, J. L. Riechmann, K. Roberg, D. Wagner, R. Williams, and E. Ziegelhoffer for critical reading of the manuscript. R. W. M. S. was supported by a long-term fellowship (LT-12/95) from the Human Frontier Science Program, and work in E. M. M.'s laboratory is supported by National Science Foundation grant MCB-9603821. The GenBank accession number for the new gene sequence reported in this paper is AJ222713.Additional details
- Eprint ID
- 72834
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- CaltechAUTHORS:20161214-153236893
- LT-12/95
- Human Frontier Science Program
- MCB-9603821
- NSF
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2016-12-15Created from EPrint's datestamp field
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2021-11-11Created from EPrint's last_modified field